plko 1 puro constructs Search Results


plko 1 puro constructs  (Addgene inc)
96
Addgene inc plko 1 puro constructs
Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+puro+(Plasmid+%238453)/pm41844497-60-9-16
Average 96 stars, based on 1 article reviews
plko 1 puro constructs - by Bioz Stars, 2026-11
96/100 stars
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shnrf2 plko 1 puro vector shrna  (Addgene inc)
95
Addgene inc shnrf2 plko 1 puro vector shrna
Shnrf2 Plko 1 Puro Vector Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1-puro+Luciferase+shRNA+Control%2C+puro+(Plasmid+%23114448)/pmc06302185-511-2-26
Average 95 stars, based on 1 article reviews
shnrf2 plko 1 puro vector shrna - by Bioz Stars, 2026-11
95/100 stars
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control scrambled shrna shc002  (Addgene inc)
85
Addgene inc control scrambled shrna shc002
Control Scrambled Shrna Shc002, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+puro+CXCR4+siRNA-2+(Plasmid+%2312272)/pmc03305999-130-46-57
Average 85 stars, based on 1 article reviews
control scrambled shrna shc002 - by Bioz Stars, 2026-11
85/100 stars
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plko.1-puro  (SymBio Inc)
90
SymBio Inc plko.1-puro
Plko.1 Puro, supplied by SymBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/plko+1+puro/pmc11005177-68-25-26
Average 90 stars, based on 1 article reviews
plko.1-puro - by Bioz Stars, 2026-11
90/100 stars
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n a recombinant dna plko 1 puro addgene addgene plasmid  (Addgene inc)
93
Addgene inc n a recombinant dna plko 1 puro addgene addgene plasmid
N A Recombinant Dna Plko 1 Puro Addgene Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pAG415-ADH1p-HA-rap1-K9R+(Plasmid+%2384531)/pm38615319-218-190-194
Average 93 stars, based on 1 article reviews
n a recombinant dna plko 1 puro addgene addgene plasmid - by Bioz Stars, 2026-11
93/100 stars
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β catenin shrna construct  (Addgene inc)
93
Addgene inc β catenin shrna construct
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
β Catenin Shrna Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+puro+shRNA+beta-catenin+(Plasmid+%2318803)/pmc08133758-284-1-7
Average 93 stars, based on 1 article reviews
β catenin shrna construct - by Bioz Stars, 2026-11
93/100 stars
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human u6 promoter plko 1  (Addgene inc)
93
Addgene inc human u6 promoter plko 1
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Human U6 Promoter Plko 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+puro-humanU6-shRNA+FASN+(Plasmid+%2382327)/pmc04040628-129-9-23
Average 93 stars, based on 1 article reviews
human u6 promoter plko 1 - by Bioz Stars, 2026-11
93/100 stars
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plko 1 puro cxcr4 sirna 1  (Addgene inc)
90
Addgene inc plko 1 puro cxcr4 sirna 1
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Plko 1 Puro Cxcr4 Sirna 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pRNATU6%2E1+HDAC3+shRNA2+(Plasmid+%23112271)/pmc03789964-185-17-42
Average 90 stars, based on 1 article reviews
plko 1 puro cxcr4 sirna 1 - by Bioz Stars, 2026-11
90/100 stars
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lentiviral plko.1-puro shrna constructs  (Broad Institute Inc)
90
Broad Institute Inc lentiviral plko.1-puro shrna constructs
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Lentiviral Plko.1 Puro Shrna Constructs, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/plko+1/pmc04280614-284-0-8
Average 90 stars, based on 1 article reviews
lentiviral plko.1-puro shrna constructs - by Bioz Stars, 2026-11
90/100 stars
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recombinant dna reagent plko 1 backbone addgene rrid addgene 30323 recombinant dna reagent pgl4 17 basic vector promega  (Addgene inc)
95
Addgene inc recombinant dna reagent plko 1 backbone addgene rrid addgene 30323 recombinant dna reagent pgl4 17 basic vector promega
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Recombinant Dna Reagent Plko 1 Backbone Addgene Rrid Addgene 30323 Recombinant Dna Reagent Pgl4 17 Basic Vector Promega, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/10__7554_slash_elife__56434-237-16-21
Average 95 stars, based on 1 article reviews
recombinant dna reagent plko 1 backbone addgene rrid addgene 30323 recombinant dna reagent pgl4 17 basic vector promega - by Bioz Stars, 2026-11
95/100 stars
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lentiviral construct plko 1 puro u6 sgrna bfuai large stuffer  (Addgene inc)
93
Addgene inc lentiviral construct plko 1 puro u6 sgrna bfuai large stuffer
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Lentiviral Construct Plko 1 Puro U6 Sgrna Bfuai Large Stuffer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1-puro+U6+sgRNA+BfuAI+large+stuffer+(Plasmid+%2352628)/pmc06615919-49-12-33
Average 93 stars, based on 1 article reviews
lentiviral construct plko 1 puro u6 sgrna bfuai large stuffer - by Bioz Stars, 2026-11
93/100 stars
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lentiviral plko 1 shrna construct shgfp  (Addgene inc)
94
Addgene inc lentiviral plko 1 shrna construct shgfp
( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA <t>(shRNA)</t> or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.
Lentiviral Plko 1 Shrna Construct Shgfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro+constructs/pLKO%2E1+puro+GFP+siRNA+(Plasmid+%2312273)/10__1158_slash_0008___5472__can___26___1063-43-1-6
Average 94 stars, based on 1 article reviews
lentiviral plko 1 shrna construct shgfp - by Bioz Stars, 2026-11
94/100 stars
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Image Search Results


( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA (shRNA) or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.

Journal: Science Advances

Article Title: Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signaling identifies therapeutic targets for colorectal cancer

doi: 10.1126/sciadv.abf2567

Figure Lengend Snippet: ( A ) Schematic diagram of the artificial TOP-GC reporter. The fluorescence of dGFP and mCherry in the reporter cells is shown in the bottom. ( B ) Validation of TOP-GC reporter responsiveness following β-catenin interference using flow cytometric analysis. Left: GFP expression was examined in parental DLD cells or TOP-GC reporter cells stably expressing nontargeting control (NTC) short hairpin–mediated RNA (shRNA) or β-catenin shRNAs. Right: FACS analysis of mCherry expression in DLD1 parental, NTC-ablated, and β-catenin–ablated DLD1 TOP-GC reporter cells. ( C ) Workflow of the screening strategy. ( D ) Volcano plot for the GFP-low hits of TOP-GC reporter screening. The x axis denotes the Z score of gene-level FC [median log 2 (fold change) for all sgRNAs per gene]. The P values in y axis were generated by MAGeCK-VISPR. ( E ) Venn diagram shows the overlaps between the hits of DLD1 TOP-GC reporter (GFP-low) and proliferation screens. ( F ) The distribution of log 2 (fold change) of sgRNAs of the entire library (top) and individual genes (bottom) in TOP-GC reporter and proliferation screenings. MAGeCK P values per gene were indicated. ( G ) Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of the expression of β-catenin targets in DLD1 and SW480 cells at day 7 after infection of lentiviruses harboring β-catenin, TCF4, LEF1, and BCL9L sgRNAs.

Article Snippet: The β-catenin shRNA construct was obtained from Addgene (#18803).

Techniques: Fluorescence, Biomarker Discovery, Expressing, Stable Transfection, Control, shRNA, Generated, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Infection